Create tile image for your game using python script

What is tile image?
Tile image is a method of storing a sequence of images placed in a single image file.
These images are then processed according to user needs.
Here's an example below:

How we can create these images?
We can use graphics editing software to create them separately.
I used Blender 3D to create separate images.
A tutorial how to do this can be found here on section Blender 3D.
After I rendered images separately and named: 0000.png , 0001.png , 0002.png , 0003.png
I created a python script to put in an tile image, see below:

import os
import PIL
from PIL import Image
from PIL import ImageDraw
o=Image.new("RGBA",(192,48))
d= ImageDraw.Draw(o)
for pic in range(0,4):
        strpic=str(pic)
        filnam="000"+strpic+".png"
        x=pic*48
        img=Image.open(filnam)
        o.paste(img,(0+x,0))
        o.save("out.png")

The script reads the image files of size 48 pixels and puts them into one image called out.png